FASCINATION ABOUT 2-FDCK HCL

The lab states fixation will bring about the dyes to leak from the cells. I feel the belief is always that fixation could damage cell membranes. I did look for a paper which describes fixation working with methanol or acetic acid, after staining by using a DCFDA derivative. DCFDA would be the reagent that stains ROS within the kit ab113851, and I t